Phospho-Beta Catenin (S33 + S37) Recombinant Rabbit Monoclonal Antibody [JE59-59] (HA721580)
Catalog# HA721580
Rabbit Monoclonal to Phospho-Beta Catenin (S33/S37)
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WB
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Human
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Rat
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Mouse
Western blot analysis of Phospho-Beta Catenin (S33 + S37) on different lysates with Rabbit anti-Phospho-Beta Catenin (S33 + S37) antibody (HA721580) at 1/1,000 dilution.
Lane 1: 293T whole cell lysate
Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate
Lane 3: C6 whole cell lysate
Lane 4: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Beta Catenin (S33 + S37) Recombinant Rabbit Monoclonal Antibody [JE59-59] (HA721580)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding ser33 and ser37 of human β-catenin.
Host
Rabbit
Modification
Phospho
Modification Site
S33/S37
Positive Control
293T treated with 100nM Calyculin A for 15 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE59-59
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 85 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Beta Catenin (S33/S37)
SYNONYMS
b-catenin antibody;Beta catenin antibody;Beta-catenin antibody;Cadherin associated protein antibody;Catenin (cadherin associated protein), beta 1, 88kDa antibody;Catenin beta 1 antibody;Catenin beta-1 antibody;CATNB antibody;CHBCAT antibody;CTNB1_HUMAN antibody;CTNNB antibody;CTNNB1 antibody;DKFZp686D02253 antibody;FLJ25606 antibody;FLJ37923 antibody;OTTHUMP00000162082 antibody;OTTHUMP00000165222 antibody;OTTHUMP00000165223 antibody;OTTHUMP00000209288 antibody;OTTHUMP00000209289 antibody
SUBCELLULAR LOCATION
Cytoplasm
FUNCTION
Catenin beta-1, also known as beta-catenin (β-catenin), is a protein that in humans is encoded by the CTNNB1 gene. Beta-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. β-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to γ-catenin, also known as plakoglobin. Beta-catenin is widely expressed in many tissues. In cardiac muscle, beta-catenin localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocytes. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular carcinoma, colorectal carcinoma, lung cancer, malignant breast tumors, ovarian and endometrial cancer. Alterations in the localization and expression levels of beta-catenin have been associated with various forms of heart disease, including dilated cardiomyopathy. β-catenin is regulated and destroyed by the beta-catenin destruction complex, and in particular by the adenomatous polyposis coli (APC) protein, encoded by the tumour-suppressing APC gene. Therefore, genetic mutation of the APC gene is also strongly linked to cancers, and in particular colorectal cancer resulting from familial adenomatous polyposis (FAP).
CITATIONS
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Niu Yilin,et al
Melatonin promotes hair regeneration by modulating the Wnt/β-catenin signalling pathway
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WB
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Mouse
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Citation
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