Phospho-BRAF (T401) Recombinant Rabbit Monoclonal Antibody [JJ08-72] (ET1701-20)
Catalog# ET1701-20
Rabbit Monoclonal to Phospho-B Raf (T401)
Tested for
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WB
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IF-Cell
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IP
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-BRAF (T401) on different lysates with Rabbit anti-Phospho-BRAF (T401) antibody (ET1701-20) at 1/2,000 dilution.
Lane 1: C6 cell lysate
Lane 2: C6 starved overnight then treated with 200nM TPA for 4 hours cell lysate
Lane 3: C6 starved overnight then treated with 200nM TPA for 4 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-BRAF (T401) on different lysates with Rabbit anti-Phospho-BRAF (T401) antibody (ET1701-20) at 1/2,000 dilution.
Lane 1: C6 cell lysate
Lane 2: C6 starved overnight then treated with 200nM TPA for 4 hours cell lysate
Lane 3: C6 starved overnight then treated with 200nM TPA for 4 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.