phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody [PS01-18] (HA721190)
Catalog# HA721190
Rabbit Monoclonal to phospho-ATR (T1989)
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WB
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IF-Cell
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Human
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with Hydroxyurea cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721190) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody [PS01-18] (HA721190)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding thr1989 of human atr.
Host
Rabbit
Modification
phospho
Modification Site
T1989
Positive Control
HeLa cell lysate, HeLa treated with Hydroxyurea cell lysate, HeLa treated with 4mM Hydroxyurea for 20 hours.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PS01-18
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 301 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
phospho-ATR (T1989)
SYNONYMS
Ataxia telangiectasia and Rad3 related antibody;Ataxia telangiectasia and Rad3-related protein antibody;ATR antibody;ATR_HUMAN antibody;FCTCS antibody;FRAP Related Protein 1 antibody;FRAP-related protein 1 antibody;FRP1 antibody;MEC1 antibody;MEC1 mitosis entry checkpoint 1 homolog antibody;Protein kinase ATR antibody;RAC3 antibody;Rad3 related protein antibody;SCKL antibody;SCKL1 antibody;Serine/threonine protein kinase ATR antibody;Serine/threonine-protein kinase ATR antibody
SUBCELLULAR LOCATION
Chromosome, Nucleus.
FUNCTION
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex.
CITATIONS
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Liu Tingting,et al
Discovery of a Meisoindigo-Derived PROTAC as the ATM Degrader: Revolutionizing Colorectal Cancer Therapy via Synthetic Lethality with ATR Inhibitors
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WB
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Human
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Citation
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