Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) Recombinant Rabbit Monoclonal Antibody [PSH14-13] (HA723603)
Catalog# HA723603
Rabbit Monoclonal to Phospho--AMPK alpha 1 / AMPK alpha 2 (T183 / T172)
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) on different lysates with Rabbit anti-Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) antibody (HA723603) at 1/2,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: NCI-H1299 treated with 0.5μM Oligomycin for 15 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C2C12 treated with 1mM AICAR for 30 minutes cell lysate
Lane 7: C6 cell lysate
Lane 8: C6 treated with 1μM Oligomycin for 30 minutes cell lysate
Lane 9: NCI-H1299 treated with 0.5μM Oligomycin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723603) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) on different lysates with Rabbit anti-Phospho-AMPK alpha 1 (T183) +AMPK alpha 2 (T172) antibody (HA723603) at 1/2,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: NCI-H1299 treated with 0.5μM Oligomycin for 15 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C2C12 treated with 1mM AICAR for 30 minutes cell lysate
Lane 7: C6 cell lysate
Lane 8: C6 treated with 1μM Oligomycin for 30 minutes cell lysate
Lane 9: NCI-H1299 treated with 0.5μM Oligomycin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723603) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.