Phospho-AKT2 (S474) Rabbit Polyclonal Antibody (HA500116)
Catalog# HA500116
Rabbit Polyclonal to Phospho-AKT2 (S474)
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
☑ Cell treatment (CT)
Western blot analysis of Phospho-AKT2 (S474) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500116, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Untreated Hela whole cell lysates
Lane 2: Hela treated with Calyculin A whole cell lysates
Lane 3: Hela treated with insulin whole cell lysates
Western blot analysis of Phospho-AKT2 (S474) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500116, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Untreated Hela whole cell lysates
Lane 2: Hela treated with Calyculin A whole cell lysates
Lane 3: Hela treated with insulin whole cell lysates