PRODUCT CODE: ET7109-13

PGAM1 Recombinant Rabbit Monoclonal Antibody [JE40-67] (ET7109-13)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2,000 dilution.<br />
 Positive control:<br />
 Lane 1: A431   <br />
        Lane 2: A549<br />
 Lane 3: Rat brain  <br />
    Lane 4: Mouse brain
  • Western blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2,000 dilution.<br />
 Positive control:<br />
 Lane 1: A431   <br />
        Lane 2: A549<br />
 Lane 3: Rat brain  <br />
    Lane 4: Mouse brain
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with PGAM1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: A431
Lane 2: A549
Lane 3: Rat brain
Lane 4: Mouse brain

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PGAM1 Recombinant Rabbit Monoclonal Antibody [JE40-67] (ET7109-13)

Immunogen

Recombinant protein within n-terminal human pgam1.

Host

Rabbit

Positive Control

A431, A549, rat brain tissue, mouse brain tissue, human liver tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE40-67

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

29 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PGAM1

SYNONYMS

BPG dependent PGAM 1 antibody; BPG dependent PGAM1 antibody; BPG-dependent PGAM 1 antibody; OTTHUMP00000059414 antibody; PGAM 1 antibody; PGAM A antibody; PGAM B antibody; PGAM-B antibody; PGAM1 antibody; PGAM1_HUMAN antibody; PGAMA antibody; PGAMB antibody; Phosphoglycerate mutase 1 antibody; Phosphoglycerate mutase 1 brain antibody; Phosphoglycerate mutase A antibody; Phosphoglycerate mutase A nonmuscle form antibody; Phosphoglycerate mutase isozyme B antibody

SEQUENCE SIMILARITIES

Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

TISSUE SPECIFICITY

Expressed in the liver and brain. Not found in the muscle.

POST-TRANSLATIONAL MODIFICATION

Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme.

SUBCELLULAR LOCATION

Cytosol. Extracellular region or secreted. Nucleus.

FUNCTION

Members of the PGAM (phosphoglycerate mutase) family of proteins are important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism. They are responsible for catalyzing the transfer of phospho groups between the carbon atoms of phosphoglycerates. In mammals there are two types of PGAM isozymes: PGAM1 (also known as PGAMB) and PGAM2 (also known as PGAMA). In the cell, PGAM1 and PGAM2 exist as either homodimers or heterodimers and are responsible for the interconversion of 3-phosphoglycerate and 2-phosphoglycerate. PGAM2 homodimers are expressed in skeletal muscle, mature sperm cells and heart; PGAM1 homodimers are found in most other tissues; and PGAM1/PGAM2 heterodimers are found exclusively in the heart. PGAM4, also known as PGAM3, is a protein formerly considered to be specific to humans. Initially the PGAM4 gene was described as a pseudogene but it is now known to encode a functional protein at least 25 million years old. The gene encoding PGAM4 is believed to have originated by retrotransposition, with the original copy being the PGAM1 gene.