Western blot analysis of PFAS on different lysates with Rabbit anti-PFAS antibody (ER1706-96) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: Hela cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: 293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 145 kDa
Observed band size: 145 kDa
Exposure time: 1 minute;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-96) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Rabbit polyclonal primary
PFAS Rabbit Polyclonal Antibody (ER1706-96)
Recombinant protein within human purl aa 1,133-1,338 / 1,338.
293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Hela cell lysate, HepG2 cell lysate, A549 cell lysate, 293 cell lysate, human tonsil tissue, human spleen tissue, human breast tissue, human kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
In the N-terminal section; belongs to the FGAMS family.
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis. Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.