PFAS Rabbit Polyclonal Antibody (ER1706-96)
Catalog# ER1706-96
Rabbit Polyclonal to PFAS
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WB
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IHC-P
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Human
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Mouse
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: Hela cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: 293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 145 kDa
Observed band size: 145 kDa
Exposure time: 1 minute;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-96) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
PFAS Rabbit Polyclonal Antibody (ER1706-96)
Immunogen
Recombinant protein within human purl aa 1,133-1,338 / 1,338.
Host
Rabbit
Positive Control
293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Hela cell lysate, HepG2 cell lysate, A549 cell lysate, 293 cell lysate, human tonsil tissue, human spleen tissue, human breast tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 145 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PFAS
SYNONYMS
PFAS antibody;FGAM synthase antibody;FGAMS antibody;FGAR amidotransferase antibody;FGARAT antibody;Formylglycinamide ribotide amidotransferase antibody;Formylglycinamide ribotide synthetase antibody;KIAA0361 antibody;Phosphoribosylformylglycinamidine synthase antibody;PURL antibody
SEQUENCE SIMILARITIES
In the N-terminal section; belongs to the FGAMS family.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis. Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
CITATIONS
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Chengming Ding,et al
PFAS promotes colorectal cancer progression via regulating RIG-I-mediated innate immune signalling
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IHC;WB
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Human
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Citation
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