Periostin Recombinant Rabbit Monoclonal Antibody [JA63-40] (ET1704-65)
Catalog# ET1704-65
Rabbit Monoclonal to Periostin
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WB
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Human
Lane 1: HaCaT cell lysate
Lane 2: HFF-1 cell lysate
Lane 3: BxPC-3 cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 85 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Periostin Recombinant Rabbit Monoclonal Antibody [JA63-40] (ET1704-65)
Immunogen
Synthetic pepride within human perilipin-1 aa 11-60 / 836.
Host
Rabbit
Positive Control
HaCaT cell lysate, HFF-1 cell lysate, BxPC-3 cell lysate, SH-SY5Y cell lysate, 293T cell lysate, human skin tissue lysate, human lung tissue lysate, human brain tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA63-40
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 93 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Periostin
SYNONYMS
Fasciclin-I like antibody;MGC119510 antibody;MGC119511 antibody;OSF 2 antibody;OSF-2 antibody;OSF2 antibody;Osteoblast specific factor 2 (fasciclin I like) antibody;Osteoblast specific factor 2 antibody;Osteoblast specific factor antibody;Osteoblast-specific factor 2 antibody;PDLPOSTN antibody;Periodontal ligament specific periostin antibody;Periostin antibody;Periostin isoform thy2 antibody;Periostin isoform thy4 antibody;Periostin isoform thy6 antibody;Periostin isoform thy8 antibody;Periostin osteoblast specific factor antibody;PN antibody;POSTN antibody;POSTN_HUMAN antibody;RP11 412K4.1 antibody
TISSUE SPECIFICITY
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
POST-TRANSLATIONAL MODIFICATION
Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues.
SUBCELLULAR LOCATION
Golgi apparatus, Secreted, extracellular space, extracellular matrix.
FUNCTION
Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta and alpha(V)beta integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor β 1(TGF β 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.
CITATIONS
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Bangwei Cao
Exosomes derived from 5-fluorouracil-resistant colon cancer cells are enriched in GDF15 and can promote angiogenesis
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WB
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Human
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Citation
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