Perforin-1 Recombinant Rabbit Monoclonal Antibody [PSH15-92] (HA723797)
Catalog# HA723797
Rabbit Monoclonal to Perforin-1
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Perforin-1 on different lysates with Rabbit anti-Perforin-1 antibody (HA723797) at 1/20,000 dilution.
Lane 1: NIH/3T3 cell lysate (negative)
Lane 2: CTLL-2 cell lysate
Lane 3: C2C12 cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 75 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723797) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Perforin-1 on different lysates with Rabbit anti-Perforin-1 antibody (HA723797) at 1/20,000 dilution.
Lane 1: NIH/3T3 cell lysate (negative)
Lane 2: CTLL-2 cell lysate
Lane 3: C2C12 cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 75 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723797) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.