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PRODUCT CODE: ET1705-99

PDX1 Recombinant Rabbit Monoclonal Antibody [JM62-45] (ET1705-99)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

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Western blot analysis of PDX1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PDX1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Immunofluorescence staining of paraffin-embedded human pancreas tissue using anti-PDX1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1705-99 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PDX1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-99, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of PDX1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PDX1 Recombinant Rabbit Monoclonal Antibody [JM62-45] (ET1705-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM62-45

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:200

  • ICC/IF

  • 1:50-1:200

  • WB

  • 1:500

TARGET

UNIPROT #

P52945

PROTEIN NAME

PDX1

SYNONYMS

Glucose sensitive factor antibody; Glucose-sensitive factor antibody; GSF antibody; IDX 1 antibody; IDX-1 antibody; IDX1 antibody; Insulin promoter factor 1 antibody; Insulin promoter factor 1 homeodomain transcription factor antibody; Insulin upstream factor 1 antibody; IPF 1 antibody; IPF-1 antibody; IPF1 antibody; Islet/duodenum homeobox 1 antibody; Islet/duodenum homeobox-1 antibody; IUF 1 antibody; IUF-1 antibody; IUF1 antibody; MODY4 antibody; Pancreas/duodenum homeobox 1 antibody; Pancreas/duodenum homeobox protein 1 antibody; pancreatic and duodenal homeobox P antibody; PDX 1 antibody; PDX-1 antibody; PDX1 antibody; PDX1_HUMAN antibody; Somatostatin transactivating factor 1 antibody; Somatostatin-transactivating factor 1 antibody; STF 1 antibody; STF-1 antibody; STF1 antibody

SEQUENCE SIMILARITIES

Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.

TISSUE SPECIFICITY

Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. Phosphorylated by HIPK2 on Ser-268 upon glucose accumulation. This phosphorylation mediates subnuclear localization shifting. Phosphorylation by PASK may lead to translocation into the cytosol (By similarity).

SUBCELLULAR LOCATION

Cytosol, Nucleus.

FUNCTION

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.