Recombinant Rabbit monoclonal primary
PDK2 Recombinant Rabbit Monoclonal Antibody [JB66-93] (ET7107-46)
Rat heart tissue lysates, rat brain tissue, human colon carcinoma tissue, human fetal skeletal muscle tissue, mouse cerebellum tissue, SH-SY5Y.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody; mitochondrial antibody; PDHK2 antibody; PDK2 antibody; PDK2_HUMAN antibody; Pyruvate dehydrogenase kinase isoform 2 antibody; Pyruvate dehydrogenase kinase, isozyme 2 antibody; Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody
Belongs to the PDK/BCKDK protein kinase family.
Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
Pyruvate dehydrogenase kinase family members (PDK1, 2, 3, 4) are serine kinases that catalyze the phosphorylation of the E1α subunit of the pyruvate dehydrogenase complex (PDC). PDC activity is controlled through phosphorylation and dephosphorylation of the E1α subunit, which leads to inactivation and reactivation, respectively. The core of PDC is composed of sixty dihydrolypoyl acetyltransferase (E2) subunits that bind directly to PDK2 and enhance PDK2 kinase activity. Upregulation of PDK isoenzymes occurs during starvation conditions, rerouting acetyl-CoA generation by facilitating fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases, in that both require Histidine residues for activity. In mammals, transcripts for PDK2 are ubiquitously expressed with high levels in heart and skeletal muscle and decreased levels in spleen and lung.