PDK1 Recombinant Rabbit Monoclonal Antibody [JA67-30] (ET1704-66)
Catalog# ET1704-66
Rabbit Monoclonal to PDK1
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WB
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IHC-P
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IP
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FC
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-66) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PDK1 Recombinant Rabbit Monoclonal Antibody [JA67-30] (ET1704-66)
Immunogen
Synthetic peptide within human pdk1 aa 16-65 / 436.
Host
Rabbit
Positive Control
Mouse heart tissue lysate, rat heart tissue lysate, Hela, NIH/3T3, mouse heart tissue, rat heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA67-30
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:2,000
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FC
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1:50-1:100
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IF-Tissue
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1:200-1:400
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 49 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PDK1
SYNONYMS
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody;HGNC:8809 antibody;Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody;PDH kinase 1 antibody;Pdk1 antibody;PDK1_HUMAN antibody;Pyruvate dehydrogenase kinase isoform 1 antibody;Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
SEQUENCE SIMILARITIES
Belongs to the PDK/BCKDK protein kinase family.
TISSUE SPECIFICITY
Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.
SUBCELLULAR LOCATION
Mitochondrion matrix.
FUNCTION
Mitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation and dephosphorylation leading to reactivation of PDH. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. PDK1 consists of alpha and beta subunits; the kinase activity resides in the alpha subunit. Three PDK isoenzymes have been identified in humans (PDK1, 2 and 3) and two have been identified in rodent (PDK1 and 2).
CITATIONS
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ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells
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Co-IP
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Human
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Citation
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He Yining,et al
Exploration of the underlying mechanism of Astragaloside III in attenuating immunosuppression via network pharmacology and vitro/vivo pharmacological validation
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WB
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Mouse
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Citation
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