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PRODUCT CODE: ER40404

PDI Rabbit Polyclonal Antibody (ER40404)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.<br /> Positive control: <br /> Lane 1: Hela&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 2: MCF-7<br /> Lane 3: PANC&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 4: HepG2<br /> Lane 5: HUVEC&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 6: NIH/3T3<br /> Lane 7: L929&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 8: F9<br /> Lane 9: Jurkat&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 10: A431<br /> Lane 11: A549&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 12: Mouse liver<br /> Lane 13: Mouse kidney&nbsp;&nbsp;&nbsp;Lane 14: Mouse brain<br /> Lane 15: Human kidney&nbsp;&nbsp;&nbsp;Lane 16: Human brain<br /> Lane 17: Human liver
  • Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.<br /> Positive control: <br /> Lane 1: Hela&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 2: MCF-7<br /> Lane 3: PANC&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 4: HepG2<br /> Lane 5: HUVEC&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 6: NIH/3T3<br /> Lane 7: L929&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 8: F9<br /> Lane 9: Jurkat&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 10: A431<br /> Lane 11: A549&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 12: Mouse liver<br /> Lane 13: Mouse kidney&nbsp;&nbsp;&nbsp;Lane 14: Mouse brain<br /> Lane 15: Human kidney&nbsp;&nbsp;&nbsp;Lane 16: Human brain<br /> Lane 17: Human liver
  • ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDI in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.
Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela     Lane 2: MCF-7
Lane 3: PANC     Lane 4: HepG2
Lane 5: HUVEC     Lane 6: NIH/3T3
Lane 7: L929     Lane 8: F9
Lane 9: Jurkat     Lane 10: A431
Lane 11: A549     Lane 12: Mouse liver
Lane 13: Mouse kidney   Lane 14: Mouse brain
Lane 15: Human kidney   Lane 16: Human brain
Lane 17: Human liver

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PDI Rabbit Polyclonal Antibody (ER40404)

Immunogen

Synthetic peptide within residues of pdi aa 476-525 / 525.

Host

Rabbit

Positive Control

Hela, MCF-7, PANC,HepG2, HUVEC, NIH/3T3, L929, F9, Jurkat, A431, A549, mouse liver tissue, mouse kidney tissue, mouse brain tissue,human lung carcinoma tissue，human kidney,Human brain,Human liver,human colon carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

Q13087

PROTEIN NAME

PDI

SYNONYMS

Pancreas specific protein disulfide isomerase antibody; Pancreas-specific protein disulfide isomerase antibody; Pancreatic protein disulfide isomerase antibody; PDA2 antibody; PDI antibody; PDIA2 antibody; PDIA2_HUMAN antibody; PDIp antibody; PDIR antibody; Protein disulfide isomerase A2 antibody; Protein disulfide isomerase antibody; Protein disulfide isomerase associated 2 antibody; Protein disulfide isomerase family A member 2 antibody; Protein disulfide isomerase pancreatic antibody; Protein disulfide-isomerase A2 antibody; Rho GDP dissociation inhibitor gamma antibody

SEQUENCE SIMILARITIES

Belongs to the protein disulfide isomerase family.

TISSUE SPECIFICITY

Highly expressed in pancreas (at protein level).

POST-TRANSLATIONAL MODIFICATION

The disulfide-linked homodimer exhibits an enhanced chaperone activity.; Glycosylated.

SUBCELLULAR LOCATION

Endoplasmic reticulum lumen

FUNCTION

Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Another major function of PDI relates to its activity as a chaperone; i.e., it aids wrongly folded proteins to reach a correctly folded state without the aid of enzymatic disulfide shuffling. PDI has been found to be involved in the breaking of bonds on the HIV gp120 protein during HIV infection of CD4 positive cells, and is required for HIV infection of lymphocytes and monocytes.

CITATIONS

  • Cao, X., Shu, Y., Chen, ......

    Cao, X., Shu, Y., Chen, Y., Xu, Q., Guo, G., Wu, Z., Shao, M., Zhou, Y., Chen, M., Gong, Y., Li, C., Shi, Y., & Bu, H. (2021). Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.