PRODUCT CODE: ET1702-70

PDI Recombinant Rabbit Monoclonal Antibody [JF97-08] (ET1702-70)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PDI on different lysates using anti-PDI antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: NIH/3T3      <br />
Lane 2: Human liver
  • Western blot analysis of PDI on different lysates using anti-PDI antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: NIH/3T3      <br />
Lane 2: Human liver
  • Flow cytometric analysis of Hela cells with PDI antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of PDI on different lysates using anti-PDI antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: Human liver

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PDI Recombinant Rabbit Monoclonal Antibody [JF97-08] (ET1702-70)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF97-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PDI

SYNONYMS

Pancreas specific protein disulfide isomerase antibody; Pancreas-specific protein disulfide isomerase antibody; Pancreatic protein disulfide isomerase antibody; PDA2 antibody; PDI antibody; PDIA2 antibody; PDIA2_HUMAN antibody; PDIp antibody; PDIR antibody; Protein disulfide isomerase A2 antibody; Protein disulfide isomerase antibody; Protein disulfide isomerase associated 2 antibody; Protein disulfide isomerase family A member 2 antibody; Protein disulfide isomerase pancreatic antibody; Protein disulfide-isomerase A2 antibody; Rho GDP dissociation inhibitor gamma antibody

SEQUENCE SIMILARITIES

Belongs to the protein disulfide isomerase family.

SUBCELLULAR LOCATION

Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Cell membrane.

FUNCTION

Oxidoreductase-protein disulfide isomerase (PDI) is a homodimer consisting of subunits that catalyzes thiol-disulfide exchange, mediates folding of newly synthesized proteins and functions as a molecular chaperone. PDI localizes to the lumen of the endoplasmic reticulum (ER), where in conjunction with folding-helper proteins, such as immunoglobulin heavy chain binding protein (BiP), mediates tertiary and quaternary protein-processing. Cell surface PDI induces sulfhydryl-mediated conformational changes in integrin-mediated adhesion receptor-ligand interactions, thereby regulating integrin responses and cell adhesion. Additionally, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins.