PRODUCT CODE: HA500019

PDCD6 Rabbit Polyclonal Antibody (HA500019)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of PDCD6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500019, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SK-Br-3 cell lysate<br />
Lane 2: HepG2 cell lysate<br />
Lane 1: Rat ovary tissue lysate
  • Western blot analysis of PDCD6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500019, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SK-Br-3 cell lysate<br />
Lane 2: HepG2 cell lysate<br />
Lane 1: Rat ovary tissue lysate
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDCD6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500019, 1/400)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PDCD6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500019, 1/400)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PDCD6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500019, 1/400)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of PDCD6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500019, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SK-Br-3 cell lysate
Lane 2: HepG2 cell lysate
Lane 1: Rat ovary tissue lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PDCD6 Rabbit Polyclonal Antibody (HA500019)

Immunogen

Recombinant protein within human pdcd6 aa 1-191.

Host

Rabbit

Positive Control

SK-Br-3 cell lysate, HepG2 cell lysate, rat ovary tissue lysate, human breast carcinoma tissue, human placenta tissue, human stomach carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

PDCD6

SYNONYMS

AIP1 antibody; ALG 2 antibody; ALG-2-interacting protein 1 antibody; ALG2 antibody; ALIX antibody; Apoptosis linked gene 2 antibody; Apoptosis linked gene 2 protein antibody; Apoptosis-linked gene 2 protein antibody; FLJ42309 antibody; FLJ46208 antibody; Hp95 antibody; KIAA1375 antibody; MA 3 antibody; MA3 antibody; MGC111017 antibody; MGC119050 antibody; MGC9123 antibody; PDCD 6 antibody; Pdcd6 antibody; PDCD6_HUMAN antibody; PEF 1B antibody; PEF1B antibody; Probable calcium binding protein ALG 2 antibody; Probable calcium binding protein ALG2 antibody; Probable calcium-binding protein ALG-2 antibody; Programmed cell death 6 antibody; Programmed cell death 6-interacting protein antibody; Programmed cell death protein 6 antibody; PS 2 antibody; PS2 antibody

SUBCELLULAR LOCATION

Endosome, Endoplasmic reticulum membrane, Nucleus, COPII-coated vesicle membrane, Cytoplasm.

FUNCTION

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is also located on the short arm of chromosome 5.