PDCD4 Recombinant Rabbit Monoclonal Antibody [JA80-83] (ET1704-70)
Catalog# ET1704-70
Rabbit Monoclonal to PDCD4
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Western blot analysis of PDCD4 on different lysates with Rabbit anti-PDCD4 antibody (ET1704-70) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PDCD4 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: 120 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PDCD4 Recombinant Rabbit Monoclonal Antibody [JA80-83] (ET1704-70)
Immunogen
Synthetic peptide within human pdcd4 aa 425-469/469.
Host
Rabbit
Positive Control
Rat pancreas tissue lysate, Daudi cell lysate, A431, human tonsil tissue, mouse colon tissue, rat lung tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA80-83
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 52 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PDCD4
SYNONYMS
Death up-regulated gene protein antibody;Dug antibody;H731 antibody;Ma3 antibody;MGC33046 antibody;MGC33047 antibody;Neoplastic transformation inhibitor antibody;Neoplastic transformation inhibitor protein antibody;Nuclear antigen H731 antibody;Nuclear antigen H731 like antibody;Nuclear antigen H731 like protein antibody;Nuclear antigen H731-like antibody;PDCD 4 antibody;Pdcd4 antibody;PDCD4_HUMAN antibody;Programmed cell death 4 antibody;programmed cell death 4 (neoplastic transformation inhibitor) antibody;Programmed cell death protein 4 antibody;Protein 197/15a antibody;Protein MA-3 antibody;Tis antibody;Topoisomerase-inhibitor suppressed protein antibody
SEQUENCE SIMILARITIES
Belongs to the PDCD4 family.
TISSUE SPECIFICITY
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
POST-TRANSLATIONAL MODIFICATION
Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.; Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4.
SUBCELLULAR LOCATION
Nucleus. Cytoplasm.
FUNCTION
The transformation suppressor gene Pdcd-4 (programmed cell death gene 4) inhibits the tumor-promoter mediated transformation of mouse keratinocytes and is a potential tumor suppressor gene in the development of human lung cancer. Biochemical analysis suggests that the Pdcd-4 protein is involved in protein translation as well as in nuclear events. Pdcd-4 directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Pdcd-4 also suppresses the transactivation of AP-1 responsive promoters by c-Jun, suggesting that the transformation-suppressor activity of Pdcd-4 might be due, at least in part, to the inhibition of c-Jun activity. In addition to affecting c-Jun phosphorylation, Pdcd-4 blocks the recruitment of the co-activator p300 by c-Jun.
CITATIONS
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Yang Yuling,et al
Exosome/antimicrobial peptide laden hydrogel wound dressings promote scarless wound healing through miR-21-5p-mediated multiple functions
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WB
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Human
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Mouse
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Citation
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