PD1 Mouse Monoclonal Antibody [1F2] (EM1707-60)
Catalog# EM1707-60
Mouse Monoclonal to PD1
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WB
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IF-Cell
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IHC-P
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FC
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Human
Western blot analysis of PD1 on PD1 transfected 293FT cell lysate using anti-PD1 antibody at 1/1,000 dilution.
Specifications
Product Type
Mouse monoclonal primary
Product Name
PD1 Mouse Monoclonal Antibody [1F2] (EM1707-60)
Immunogen
Recombinant protein within human pd1 aa 1-200 / 288.
Host
Mouse
Positive Control
Human tonsils tissue, Jurkat, recombinant PD1 protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
1F2
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:100-1:500
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 32 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
PD1
SYNONYMS
CD279 antibody;CD279 antigen antibody;hPD 1 antibody;hPD l antibody;hPD-1 antibody;hSLE1 antibody;PD 1 antibody;PD-1 antibody;PD1 antibody;PDCD 1 antibody;PDCD1 antibody;PDCD1_HUMAN antibody;Programmed cell death 1 antibody;Programmed cell death 1 protein antibody;Programmed cell death protein 1 antibody;Protein PD 1 antibody;Protein PD-1 antibody;SLEB2 antibody;Systemic lupus erythematosus susceptibility 2 antibody
DEVELOPMENTAL STAGE
Induced at programmed cell death.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains.; Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.; N-glycosylation at Asn-58 consists of two N-acetylglucosamine units and one fucose. N-glycosylation does not affect binding to nivolumab drug.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Pdcd-1 (Programmed Cell Death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. Pdcd-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) within the cytoplasmic domain, which is conserved between the mouse and human homologs. Expression of Pdcd-1 is detected in mouse thymus, and it is induced in stimulated B and T cell lines, where it may play a role in the negative regulation of various immune responses. Receptors such as Pdcd-1 function by recruiting tyrosine phosphatases, including SHP-1 and SHIP, which are responsible for altering various B cell responses. Additionally, in activated lymphocytes, Pdcd-1 mediates the activation of the classical type of programmed cell death.
CITATIONS
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Cen, S., Liu, K., Zheng, Y., Shan, J., Jing, C., Gao, J., Pan, H., Bai, Z., & Liu, Z.
BRAF Mutation as a Potential Therapeutic Target for Checkpoint Inhibitors: A Comprehensive Analysis of Immune Microenvironment in BRAF Mutated Colon Cancer
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IHC
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Human
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Citation
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