Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
PD1 Mouse Monoclonal Antibody [1F2] (EM1707-60)
Immunogen
Recombinant protein
Host
Mouse
Positive Control
Human tonsil tissue, Mouse spleen tissue, Jurkat, recombinant PD1 protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
1F2
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
32 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:100-1:500
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
PD1
SYNONYMS
CD279 antibody; CD279 antigen antibody; hPD 1 antibody; hPD l antibody; hPD-1 antibody; hSLE1 antibody; PD 1 antibody; PD-1 antibody; PD1 antibody; PDCD 1 antibody; PDCD1 antibody; PDCD1_HUMAN antibody; Programmed cell death 1 antibody; Programmed cell death 1 protein antibody; Programmed cell death protein 1 antibody; Protein PD 1 antibody; Protein PD-1 antibody; SLEB2 antibody; Systemic lupus erythematosus susceptibility 2 antibody
DEVELOPMENTAL STAGE
Induced at programmed cell death.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains.; Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.; N-glycosylation at Asn-58 consists of two N-acetylglucosamine units and one fucose. N-glycosylation does not affect binding to nivolumab drug.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Pdcd-1 (Programmed Cell Death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. Pdcd-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) within the cytoplasmic domain, which is conserved between the mouse and human homologs. Expression of Pdcd-1 is detected in mouse thymus, and it is induced in stimulated B and T cell lines, where it may play a role in the negative regulation of various immune responses. Receptors such as Pdcd-1 function by recruiting tyrosine phosphatases, including SHP-1 and SHIP, which are responsible for altering various B cell responses. Additionally, in activated lymphocytes, Pdcd-1 mediates the activation of the classical type of programmed cell death.