PCTAIRE1 Recombinant Rabbit Monoclonal Antibody [JE64-55] (HA721964)
Catalog# HA721964
Rabbit Monoclonal to PCTAIRE1
Tested for
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WB
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PCTAIRE1 on different lysates with Rabbit anti-PCTAIRE1 antibody (HA721964) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat pancreas tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56/63 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721964) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat pancreas tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56/63 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721964) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.