PCSK9 Recombinant Rabbit Monoclonal Antibody [JE30-37] (HA722528)
Catalog# HA722528
Rabbit Monoclonal to PCSK9
Tested for
-
WB
-
IHC-P
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA722528) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (negative)
Lane 2: Huh7 cell lysate
Lane 3: BxPC-3 cell lysate
Lane 4: HeLa cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 60/74 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722528) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA722528) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (negative)
Lane 2: Huh7 cell lysate
Lane 3: BxPC-3 cell lysate
Lane 4: HeLa cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 60/74 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722528) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.