PRODUCT CODE: EM111201

PCNA Mouse Monoclonal Antibody [A6-G11] (EM111201)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution.<br />
Positive control:    <br />
Line 1: L929    <br />
Line 2 :MCF-7  <br />
Line 3:PC12    <br />
Line 4:Raji  <br />
Line 5:F9       <br />
Line 6:A549
  • Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution.<br />
Positive control:    <br />
Line 1: L929    <br />
Line 2 :MCF-7  <br />
Line 3:PC12    <br />
Line 4:Raji  <br />
Line 5:F9       <br />
Line 6:A549
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PCNA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-PCNA antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with PCNA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution.
Positive control:
Line 1: L929
Line 2 :MCF-7
Line 3:PC12
Line 4:Raji
Line 5:F9
Line 6:A549

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PCNA Mouse Monoclonal Antibody [A6-G11] (EM111201)

Immunogen

Recombinant protein within human pcna aa 1-261.

Host

Mouse

Positive Control

L929, MCF-7, PC12, Raji, F9, A549, Hela ,human tonsil tissue, human gastric tumor tissue, mouse large intestine, mouse spleen tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A6-G11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

29 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:200

  • FC:1:100

TARGET

UNIPROT #

PROTEIN NAME

PCNA

SYNONYMS

ATLD2 antibody; cb16 antibody; Cyclin antibody; DNA polymerase delta auxiliary protein antibody; etID36690.10 antibody; fa28e03 antibody; fb36g03 antibody; HGCN8729 antibody; MGC8367 antibody; Mutagen-sensitive 209 protein antibody; OTTHUMP00000030189 antibody; OTTHUMP00000030190 antibody; PCNA antibody; Pcna/cyclin antibody; PCNA_HUMAN antibody; PCNAR antibody; Polymerase delta accessory protein antibody; Proliferating cell nuclear antigen antibody; wu:fa28e03 antibody; wu:fb36g05 antibody

SEQUENCE SIMILARITIES

Belongs to the PCNA family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.; Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation. Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner. Acetylation disrupts interaction with NUDT15 and promotes degradation.; Ubiquitinated. Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase.; Methylated on glutamate residues by ARMT1/C6orf211.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

PCNA (Proliferating cell nuclear antigen) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities.