PRODUCT CODE: ET7107-29

PCK2 Recombinant Rabbit Monoclonal Antibody [JB52-39] (ET7107-29)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.
  • Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.
  • ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PCK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PCK2 Recombinant Rabbit Monoclonal Antibody [JB52-39] (ET7107-29)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat kidney tissue, human liver tissue, human colon cancer tissue, mouse colon tissue, human kidney tissue, MCF-7, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB52-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

71 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PCK2

SYNONYMS

EC 4.1.1.32 antibody; GTP mitochondrial precursor antibody; HGNC:8725 antibody; mitochondrial antibody; Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody; OTTHUMP00000164700 antibody; PCK2 antibody; PCKGM_HUMAN antibody; PE antibody; PEP carboxykinase antibody; PEPCK antibody; PEPCK deficiency mitochondrial antibody; PEPCK M antibody; PEPCK-M antibody; PEPCK2 antibody; Phosphoenolpyruvate carboxykinase [GTP] antibody; Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody; Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody; Phosphoenolpyruvate carboxylase antibody; Phosphopyruvate carboxylase antibody

SEQUENCE SIMILARITIES

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

Normal adjustment to changes in blood glucose levels depends on Insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while Insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken.