PCK1 Recombinant Rabbit Monoclonal Antibody [JU84-39] (ET7106-81)

UNIPROT #

P35558

PROTEIN NAME

PCK1

SYNONYMS

cytosolic [GTP] antibody;GTP antibody;PCK1 antibody;PCKGC_HUMAN antibody;PEP carboxykinase antibody;PEPCK-C antibody;PEPCK1 antibody;PEPCKC antibody;Phosphoenolpyruvate carboxykinase 1 (soluble) antibody;Phosphoenolpyruvate carboxykinase 1 antibody;Phosphoenolpyruvate carboxykinase antibody;Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody;Phosphoenolpyruvate carboxykinase, cytosolic antibody;Phosphoenolpyruvate carboxylase antibody;Phosphopyruvate carboxylase antibody

SEQUENCE SIMILARITIES

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

TISSUE SPECIFICITY

Major sites of expression are liver, kidney and adipocytes.

POST-TRANSLATIONAL MODIFICATION

Acetylated. Lysine acetylation by p300/EP300 is increased on high glucose conditions. Lysine acetylation promotes ubiquitination by UBR5. Acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2. Deacetylation of Lys-91 is carried out by SIRT1 and depends on PCK1 phosphorylation levels.; Phosphorylated in a GSK3B-mediated pathway; phosphorylation affects the efficiency of SIRT1-mediated deacetylation, and regulates PCK1 ubiquitination and degradation.; Ubiquitination by UBR5 leads to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Normal adjustment to changes in blood glucose levels depends on insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken.