Positive control:
Lane 1: 293T cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: HepG2 cell lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PBR Recombinant Rabbit Monoclonal Antibody [SA90-03] (ET1601-19)
Immunogen
Synthetic peptide within c-terminal human pbr.
Host
Rabbit
Positive Control
293T cell lysate, NIH/3T3 cell lysate, HepG2 cell lysate, Hela, PC-3M, SW480, human colon carcinoma tissue, human kidney tissue, mouse testis tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA90-03
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
19 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:5,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
-
IP
-
assay-dependent
TARGET
UNIPROT #
PROTEIN NAME
PBR
SYNONYMS
Benzodiazapine receptor (peripheral) antibody; Benzodiazepine peripheral binding site antibody; BPBS antibody; BZRP antibody; DBI antibody; IBP antibody; Isoquinoline carboxamide-binding protein antibody; MBR antibody; mDRC antibody; Mitochondrial benzodiazepine receptor antibody; PBR antibody; PBS antibody; Peripheral benzodiazepine receptor antibody; Peripheral benzodiazepine receptor-related protein antibody; Peripheral type benzodiazepine receptor antibody; Peripheral-type benzodiazepine receptor antibody; pk18 antibody; PKBS antibody; PTBR antibody; Ptbzr antibody; PTBZR02 antibody; RATPTBZR02 antibody; translocator protein (18kDa) antibody; Translocator protein antibody; Tspo antibody; Tspo1 antibody; TSPOA_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the TspO/BZRP family.
TISSUE SPECIFICITY
Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
SUBCELLULAR LOCATION
Mitochondrion membrane
FUNCTION
Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an indispensable element of the steroidogenic machinery, where it mediates the delivery of cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation. PBR is composed of three subunits, an isoquinoline binding site, a voltage-dependent anion channel and an adenine nucleotide carrier. PBR is genetically conserved from bacteria to humans and in humans is widely expressed in peripheral organs, whereas in the brain, it is sparse and located mainly in glial cells. Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits the Leydig cell steroidogenesis by affecting PBR mRNA stability, thus inhibiting PBR expression, cholesterol transport into the mitochondria and subsequent steroid formation. A cytoplasmic protein, PRAX-1 ( peripheral benzodiazepine receptor-associated protein 1), is found to specifically interact with PBR. The polypeptide diazepam binding inhibitor is an endogenous PBR ligand. PBR also binds Ro 5-4864 (4’-chlorodiazepam) and PK 11185 (an isoquinoline carboxamide derivative), but not clonazepam, and PBR regulates the cholesterol transport that results in decreased circulating corticosterone levels.