PAX8 Recombinant Rabbit Monoclonal Antibody [JJ08-88] (ET1701-50)
Catalog# ET1701-50
Rabbit Monoclonal to PAX8
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Lysates/proteins at 10 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 20 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-50) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PAX8 Recombinant Rabbit Monoclonal Antibody [JJ08-88] (ET1701-50)
Immunogen
Recombinant protein within human pax8 aa 101-450 / 450.
Host
Rabbit
Positive Control
SKOV-3 cell lysates, NIH: OVCAR-3 cell lysates, SKOV-3, NIH:OVCAR-3, human ovarian carcinoma tissue, human thyroid cancer tissue, human thyroid tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ08-88
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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IF-Tissue
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1:50-1:200
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IHC-P
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PAX8
SYNONYMS
OTTHUMP00000158659 antibody;OTTHUMP00000158660 antibody;OTTHUMP00000203723 antibody;OTTHUMP00000203724 antibody;Paired box 8 antibody;Paired box gene 8 antibody;paired box homeotic gene 8 antibody;Paired box protein Pax 8 antibody;Paired box protein Pax-8 antibody;Paired domain gene 8 antibody;PAX 8 antibody;PAX8 antibody;PAX8_HUMAN antibody
TISSUE SPECIFICITY
Expressed in the excretory system, thyroid gland and Wilms tumors.
DEVELOPMENTAL STAGE
In developing excretory system, during thyroid differentiation and in adult thyroid.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
PAX8 is a transcription factor crucial to the organogenesis and development of the thyroid gland, urogenital tract, placenta and inner ear. In the thyroid, PAX8 is a master gene that regulates maintenance of the differentiated thyroid follicular cell phenotype, where it controls and activates the transcription of the main proteins responsible for the functional activity of follicular cells such as thyroglobulin, thyroperoxidase and sodium/iodide symporter. In the developing kidney PAX8 is important for renal vescicle formation. PAX8 regulates the expression of the WT1 gene. PAX8 appears to be currently the most sensitive and specific marker for renal cell carcinoma and ovarian non-mucinous carcinoma. Follicular and papillary thyroid carcinoma are virually always PAX8 positive (while anaplastic carcinoma is positive in most cases and medullary thyroid carcinoma negative). PAX8 is also found in almost all cases of ovarian serous, endometrioid, transitional and clear cell carcinoma (while mucinous carcinoma is positive in a minor number of cases), and endometrial carcinoma.
CITATIONS
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Genome-wide alternation and effect of DNA methylation in the impairments of steroidogenesis and spermatogenesis after PM2. 5 exposure
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WB
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Mouse
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Citation
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