PARP1 Rabbit Polyclonal Antibody (ER1802-67)
Catalog# ER1802-67
Rabbit Polyclonal to PARP1
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WB
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IF-Cell
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FC
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Human
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Mouse
Specifications
Product Type
Rabbit polyclonal primary
Product Name
PARP1 Rabbit Polyclonal Antibody (ER1802-67)
Immunogen
Synthetic peptide within parp1 aa 190-270.
Host
Rabbit
Positive Control
Daudi, 293, A431, SH-SY5Y, K562.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500
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IF-Cell
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1:50-1:100
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 113 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PARP1
SYNONYMS
ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody;ADP ribosyltransferase antibody;ADP ribosyltransferase diphtheria toxin like 1 antibody;ADP ribosyltransferase NAD(+) antibody;ADPRT 1 antibody;ADPRT antibody;ADPRT1 antibody;ARTD1 antibody;msPARP antibody;NAD(+) ADP ribosyltransferase 1 antibody;NAD(+) ADP-ribosyltransferase 1 antibody;pADPRT 1 antibody;pADPRT1 antibody;PARP 1 antibody;PARP antibody;PARP-1 antibody;PARP1 antibody;PARP1_HUMAN antibody;Poly (ADP ribose) polymerase 1 antibody;poly (ADP ribose) polymerase family, member 1 antibody;Poly [ADP-ribose] polymerase 1 antibody Poly(ADP ribose) polymerase antibody;poly(ADP ribose) synthetase antibody;poly(ADP ribosyl)transferase antibody;Poly[ADP ribose] synthetase 1 antibody;Poly[ADP-ribose] synthase 1 antibody;PPOL antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.
SUBCELLULAR LOCATION
Nucleus, nucleolus, Cytoplasm, cytosol.
FUNCTION
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
CITATIONS
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Zeng HH, Ma M, Wang YL, Chen MH, Huang DB
Hyperoside attenuates carbon tetrachloride-induced hepatic fibrosis via the poly (ADP-ribose) polymerase-1-high mobility group protein 1 pathway
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WB
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Mouse
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Citation
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