PAR2 Recombinant Rabbit Monoclonal Antibody [JE35-05] (HA721426)
Catalog# HA721426
Rabbit Monoclonal to PAR2
Tested for
-
WB
-
FC
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody (HA721426) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: PANC-1 cell lysate (15 µg/Lane)
Lane 4: PC-3M cell lysate (15 µg/Lane)
Lane 5: AGS cell lysate (15 µg/Lane)
Lane 6: SGC-7901 cell lysate (15 µg/Lane)
Lane 7: Human kidney tissue lysate (30 µg/Lane)
Lane 8: Human stomach tissue lysate (30 µg/Lane)
Lane 9: Mouse large intestine tissue lysate (30 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 55 kDa (Glycosylation)
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721426) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116 cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: PANC-1 cell lysate (15 µg/Lane)
Lane 4: PC-3M cell lysate (15 µg/Lane)
Lane 5: AGS cell lysate (15 µg/Lane)
Lane 6: SGC-7901 cell lysate (15 µg/Lane)
Lane 7: Human kidney tissue lysate (30 µg/Lane)
Lane 8: Human stomach tissue lysate (30 µg/Lane)
Lane 9: Mouse large intestine tissue lysate (30 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 55 kDa (Glycosylation)
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721426) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.