Positive control:
Lane 1: human heart tissue lysate
Lane 2: mouse heart tissue lysate
Lane 3: rat heart tissue lysate
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Pan-Cadherin Recombinant Rabbit Monoclonal Antibody [ST54-01] (ET1609-70)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Human heart tissue lysate, mouse heart tissue lysate, rat heart tissue lysate, Hela, mouse liver tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST54-01
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
140 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Pan-Cadherin
SYNONYMS
Cadherin antibody; CDH3 antibody; CDHP antibody; 7B4 antigen antibody; Cadherin-1 antibody; Cadherin-2 antibody; Cadherin-3 antibody; Cadherin-4 antibody; Cadherin-5 antibody; CAM 120/80 antibody; CD144 antibody; CD324 antibody; CD325 antibody; Cdh4 antibody; CDH5 antibody; CDHE antibody; CDHN antibody; E-cadherin antibody; Epithelial cadherin antibody; N-cadherin antibody; NCAD antibody; Neural cadherin antibody; P-cadherin antibody; Placental cadherin antibody; UVO antibody; Uvomorulin antibody; Vascular endothelial cadherin antibody
TISSUE SPECIFICITY
Non-neural epithelial tissues.
POST-TRANSLATIONAL MODIFICATION
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation (By similarity).; N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location.; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754.; O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Thr-285 and Thr-509 are O-mannosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3.
SUBCELLULAR LOCATION
Cell membrane, Cell junction, Endosome, Golgi apparatus.
FUNCTION
Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin, R-cadherin, B-cadherin, E/P cadherin and cadherin-5.