Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)
Catalog# HA600032
Mouse Monoclonal to Pan-Actin
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WB
-
IHC-P
-
IF-Cell
-
Human
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Mouse
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Rat
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse smooth muscle tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 9 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)
Immunogen
Synthetic peptide corresponding to n terminal of human alpha actin (smooth muscle, skeletal muscle, cardiac muscle).
Host
Mouse
Positive Control
A431 cell lysate, MCF7 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse smooth muscle tissue lysate, rat heart tissue lysate, HeLa, human heart tissue, human skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A2E1
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
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IHC-P
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1:10,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 42 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Pan-Actin
SYNONYMS
a actin antibody;ACTA antibody;ACTA1 antibody;Actin, alpha skeletal muscle antibody;ACTS_HUMAN antibody;Alpha actin 1 antibody;Alpha-actin-1 antibody;ASMA antibody;MPFD antibody;AAT6 antibody;ACTA_HUMAN antibody;ACTA2 antibody;Actin alpha 2 smooth muscle aorta antibody;Actin aortic smooth muscle antibody;Actin, aortic smooth muscle antibody;ACTSA antibody;ACTVS antibody;Alpha 2 actin antibody;Alpha actin 2 antibody;Alpha cardiac actin antibody;alpha sma antibody;Alpha-actin-2 antibody;Cell growth inhibiting gene 46 protein antibody;Cell growth-inhibiting gene 46 protein antibody;GIG46 antibody;Growth inhibiting gene 46 antibody;MYMY5 antibody;ACTC antibody;ACTC_HUMAN antibody;ACTC1 antibody;Actin alpha cardiac muscle 1 antibody;Actin antibody;alpha cardiac muscle 1 antibody;Alpha-cardiac actin antibody;ASD5 antibody;CMD1R antibody;CMH11 antibody;LVNC4 antibody
SUBCELLULAR LOCATION
Cytoplasm, Cytoskeleton
FUNCTION
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTC1 encodes cardiac muscle alpha actin. This isoform differs from the alpha actin that is expressed in skeletal muscle, ACTA1. Alpha cardiac actin is the major protein of the thin filament in cardiac sarcomeres, which are responsible for muscle contraction and generation of force to support the pump function of the heart. ACTA2 (actin alpha 2) is an actin protein with several aliases including alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actins are a family of globular multi-functional proteins that form microfilaments. ACTA2 is one of 6 different actin isoforms and is involved in the contractile apparatus of smooth muscle. ACTA2 (as with all the actins) is extremely highly conserved and found in nearly all mammals. Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
