Palladin Recombinant Rabbit Monoclonal Antibody [JE66-56] (HA721440)
Catalog# HA721440
Rabbit Monoclonal to Palladin
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Palladin on different lysates with Rabbit anti-Palladin antibody (HA721440) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15µg/Lane)
Lane 2: SiHa cell lysate (10µg/Lane)
Lane 3: A431 cell lysate (15 µg/Lane)
Lane 4: SW480 cell lysate (30 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 8: MDA-MB-468 cell lysate (20 µg/Lane)
Lane 9: MCF7 cell lysate (20 µg/Lane)
Predicted band size: 151 kDa
Observed band size: 110/151 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721440) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (15µg/Lane)
Lane 2: SiHa cell lysate (10µg/Lane)
Lane 3: A431 cell lysate (15 µg/Lane)
Lane 4: SW480 cell lysate (30 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 8: MDA-MB-468 cell lysate (20 µg/Lane)
Lane 9: MCF7 cell lysate (20 µg/Lane)
Predicted band size: 151 kDa
Observed band size: 110/151 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721440) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.