PRODUCT CODE: ET7111-35

PAK3 Recombinant Rabbit Monoclonal Antibody [JE57-39] (ET7111-35)

  • Recombinant

Applications

  • WB

  • ICC

REACTIVITY

  • Mouse

  • Rat

  • Human

Western blot analysis of PAK3 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PAK3 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of PAK3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7111-35, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of PAK3 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

REACTIVITY

  • Mouse

  • Rat

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PAK3 Recombinant Rabbit Monoclonal Antibody [JE57-39] (ET7111-35)

Immunogen

Synthetic peptide within n-terminal human pak3.

Host

Rabbit

Positive Control

Rat brain tissue lysates, F9.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE57-39

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PAK3

SYNONYMS

Beta PAK antibody; bPAK antibody; CDKN1A antibody; hPAK3 antibody; Mental retardation X linked 30 antibody; MRX30 antibody; MRX47 antibody; Oligophrenin 3 antibody; OPHN3 antibody; p21 (CDKN1A) activated kinase 3 antibody; p21 activated kinase 3 antibody; p21 CDKN1A activated kinase 3 antibody; P21 protein (Cdc42/Rac) activated kinase 3 antibody; PAK-3 antibody; PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 antibody; PAK3beta antibody; Pak65alpha antibody; Pak65beta antibody; Serine threonine protein kinase PAK 3 antibody; Stk4 antibody

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified.