Lane 1: MCF-7 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: rat skin tissue lysate
Recombinant Rabbit monoclonal primary
PABPN1 Recombinant Rabbit Monoclonal Antibody [JM11-28] (ET1703-66)
MCF-7 cell lysate, SH-SY5Y cell lysate, rat skin tissue lysate, AGS, SW480, mouse testis tissue, Hela.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
Nuclear poly(A)-binding protein 1 antibody; OPMD antibody; PAB2 antibody; PABII antibody; PABP 2 antibody; pABP-2 antibody; PABP2 antibody; PABP2_HUMAN antibody; PABPII antibody; Pabpn1 antibody; poly(A) binding protein nuclear 1 antibody; Poly(A)-binding protein 2 antibody; Poly(A)-binding protein II antibody; PolyA binding protein II antibody; Polyadenylate-binding nuclear protein 1 antibody; Polyadenylate-binding protein 2 antibody
Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties (By similarity).
Nucleus, Nucleus speckle. Cytoplasm.
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.