p54nrb/NONO Recombinant Rabbit Monoclonal Antibody [JG87-38] (ET7108-81)
Catalog# ET7108-81
Rabbit Monoclonal to p54nrb
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Western blot analysis of p54nrb/NONO on different lysates with Rabbit anti-p54nrb/NONO antibody (ET7108-81) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si p54nrb/NONO cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 60 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-81) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
p54nrb/NONO Recombinant Rabbit Monoclonal Antibody [JG87-38] (ET7108-81)
Immunogen
Synthetic peptide within human p54nrb aa 365-471 / 471.
Host
Rabbit
Positive Control
A549 cell lysate, 293cell lysate, SH-SY-5Y cell lysate, SiHa, human liver tissue, mouse epididymis tissue, mouse testis tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG87-38
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
p54nrb
SYNONYMS
52 kDa subunit antibody;54 kDa nuclear RNA and DNA binding protein antibody;54 kDa nuclear RNA- and DNA-binding protein antibody;55 kDa nuclear protein antibody;DNA binding p52/p100 complex 52 kDa subunit antibody;DNA-binding p52/p100 complex antibody;NMT 55 antibody;NMT55 antibody;Non Pou domain containing octamer (ATGCAAAT) binding protein antibody;Non POU domain containing octamer binding antibody;Non POU domain containing octamer binding protein antibody;Non-POU domain-containing octamer-binding protein antibody;Nono antibody;NonO protein antibody;NONO_HUMAN antibody;NRB 54 antibody;NRB antibody;NRB54 antibody;Nuclear RNA binding protein 54kD antibody;P54 antibody;p54(nrb) antibody;p54nrb antibody;PPP1R114 antibody;Protein phosphatase 1 regulatory subunit 114 antibody
TISSUE SPECIFICITY
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
POST-TRANSLATIONAL MODIFICATION
The N-terminus is blocked.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.
CITATIONS
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Qixiang Li,et al
NONO directs PKM2-mediated H3T11ph to promote triple-negative breast cancer metastasis by activating SERPINE1 expression
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IF
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IHC
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Human
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Citation
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