Recombinant Rabbit monoclonal primary
p54nrb Recombinant Rabbit Monoclonal Antibody [JG87-38] (ET7108-81)
Recombinant protein of c-terminal human p54nrb.
293, SH-SY-5Y, SiHa, rat kidney tissue, human liver tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
52 kDa subunit antibody; 54 kDa nuclear RNA and DNA binding protein antibody; 54 kDa nuclear RNA- and DNA-binding protein antibody; 55 kDa nuclear protein antibody; DNA binding p52/p100 complex 52 kDa subunit antibody; DNA-binding p52/p100 complex antibody; NMT 55 antibody; NMT55 antibody; Non Pou domain containing octamer (ATGCAAAT) binding protein antibody; Non POU domain containing octamer binding antibody; Non POU domain containing octamer binding protein antibody; Non-POU domain-containing octamer-binding protein antibody; Nono antibody; NonO protein antibody; NONO_HUMAN antibody; NRB 54 antibody; NRB antibody; NRB54 antibody; Nuclear RNA binding protein 54kD antibody; P54 antibody; p54(nrb) antibody; p54nrb antibody; PPP1R114 antibody; Protein phosphatase 1 regulatory subunit 114 antibody
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
The N-terminus is blocked.
Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.