PRODUCT CODE: ET7108-81

p54nrb Recombinant Rabbit Monoclonal Antibody [JG87-38] (ET7108-81)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution.
  • Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution.
  • ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- p54nrb antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- p54nrb antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- p54nrb antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-p54nrb antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- p54nrb antibody. Counter stained with hematoxylin.
Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

p54nrb Recombinant Rabbit Monoclonal Antibody [JG87-38] (ET7108-81)

Immunogen

Recombinant protein of c-terminal human p54nrb.

Host

Rabbit

Positive Control

293, SH-SY-5Y, SiHa, rat kidney tissue, human liver tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG87-38

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50 IHC-P

TARGET

UNIPROT #

PROTEIN NAME

p54nrb

SYNONYMS

52 kDa subunit antibody; 54 kDa nuclear RNA and DNA binding protein antibody; 54 kDa nuclear RNA- and DNA-binding protein antibody; 55 kDa nuclear protein antibody; DNA binding p52/p100 complex 52 kDa subunit antibody; DNA-binding p52/p100 complex antibody; NMT 55 antibody; NMT55 antibody; Non Pou domain containing octamer (ATGCAAAT) binding protein antibody; Non POU domain containing octamer binding antibody; Non POU domain containing octamer binding protein antibody; Non-POU domain-containing octamer-binding protein antibody; Nono antibody; NonO protein antibody; NONO_HUMAN antibody; NRB 54 antibody; NRB antibody; NRB54 antibody; Nuclear RNA binding protein 54kD antibody; P54 antibody; p54(nrb) antibody; p54nrb antibody; PPP1R114 antibody; Protein phosphatase 1 regulatory subunit 114 antibody

TISSUE SPECIFICITY

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

POST-TRANSLATIONAL MODIFICATION

The N-terminus is blocked.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.