P4HB Recombinant Rabbit Monoclonal Antibody [JE54-98] (ET7110-92)
Catalog# ET7110-92
Rabbit Monoclonal to P4HB
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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Monkey
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: COS-1 cell lysate (20 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-92) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
P4HB Recombinant Rabbit Monoclonal Antibody [JE54-98] (ET7110-92)
Immunogen
Recombinant protein within human p4hb aa 360-508 / 508.
Host
Rabbit
Positive Control
THP-1 cell lysate, HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HepG2, NIH/3T3, PC-12, human liver tissue, human placenta tissue, human pancreas tissue, mouse colon tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE54-98
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:10,000
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IF-Cell
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1:250
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IHC-P:1:50-1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
P4HB
SYNONYMS
Cellular thyroid hormone binding protein antibody;Cellular thyroid hormone-binding protein antibody;Collagen prolyl 4 hydroxylase beta antibody;Disulphide Isomerase antibody;DSI antibody;EC 5.3.4.1 antibody;Endoplasmic reticulum resident protein 59 antibody;ER protein 59 antibody;ERBA2L antibody;ERp59 antibody;GIT antibody;Gltathione insulin transhydrogenase antibody;Glutathione insulin transhydrogenase antibody;P4HB antibody;P4Hbeta antibody;p55 antibody;PDI antibody;PDIA1 antibody;PDIA1_HUMAN antibody;PDIR antibody;PHDB antibody;PO4DB antibody;PO4HB antibody;Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) antibody;Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit antibody;PROHB antibody;Prolyl 4 hydroxylase beta polypeptide antibody;Prolyl 4 hydroxylase beta subunit antibody;Prolyl 4 hydroxylase subunit beta antibody;Prolyl 4-hydroxylase subunit beta antibody;Protein disulfide isomerase associated 1 antibody;Protein disulfide isomerase, family A, member 1 antibody;Protein disulfide isomerase/oxidoreductase antibody;Protein disulfide-isomerase antibody;Protocollagen hydroxylase antibody;Thbp antibody;Thyroid hormone binding protein p55 antibody;Thyroid hormone binding protein p55 cellular antibody;V erb a avian erythroblastic leukemia viral oncogene homolog 2 like antibody
SEQUENCE SIMILARITIES
Belongs to the protein disulfide isomerase family.
SUBCELLULAR LOCATION
Endoplasmic reticulum, endoplasmic reticulum lumen, cell membrane, melanosome.
FUNCTION
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
CITATIONS
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Zhang Bei,et al
Unveiling a new strategy for PDIA1 inhibition: Integration of activity-based probes profiling and targeted degradation
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WB
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Human
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Citation
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