p47-phox Mouse Monoclonal Antibody [A6B11] (HA600049)
Catalog# HA600049
Mouse Monoclonal to p47-phox
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
Specifications
Product Type
Mouse monoclonal primary
Product Name
p47-phox Mouse Monoclonal Antibody [A6B11] (HA600049)
Immunogen
Recombinant protein within human p47-phox aa 51-250.
Host
Mouse
Positive Control
Daudi cell lysates, Hela, NIH/3T3, human lung tissue, human spleen tissue, Daudi.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A6B11
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:1,000
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FC
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
p47-phox
SYNONYMS
47 kDa autosomal chronic granulomatous disease protein antibody;47 kDa neutrophil oxidase factor antibody;NADPH oxidase organizer 2 antibody;NCF 47K antibody;NCF-1 antibody;NCF-47K antibody;Ncf1 antibody;NCF1_HUMAN antibody;Neutrophil cytosol factor 1 antibody;Neutrophil cytosolic factor 1 antibody;neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody;Neutrophil NADPH oxidase factor 1 antibody;Nox organizer 2 antibody;Nox organizing protein 2 antibody;Nox-organizing protein 2 antibody;NOXO2 antibody;p47 phox antibody;p47-phox antibody;SH3 and PX domain containing protein 1A antibody;SH3 and PX domain-containing protein 1A antibody;SH3PXD1A antibody
SUBCELLULAR LOCATION
Cytoplasm, cytosol, Membrane.
FUNCTION
The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47phox and p67-phox. The cytosolic proteins p47phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47phox, suggesting that p47phox may faciliate the transport of p67-phox to the membrane.
CITATIONS
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Mengxin Cheng,et al
Qingke Pingchuan granules alleviate airway inflammation in COPD exacerbation by inhibiting neutrophil extracellular traps in mice
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WB
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mice
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Citation
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