p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-58] (HA722017)
Catalog# HA722017
Rabbit Monoclonal to p38 gamma/MAPK12
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 gamma/MAPK12 antibody (HA722017) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse heart tissue lysate
Lane 7: Mouse skeletal muscle tissue lysate
Lane 8: Rat skeletal muscle tissue lysate
Lane 9: Rat heart tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722017) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse heart tissue lysate
Lane 7: Mouse skeletal muscle tissue lysate
Lane 8: Rat skeletal muscle tissue lysate
Lane 9: Rat heart tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722017) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.