P2X7 Rabbit Polyclonal Antibody (ER1901-99)
Catalog# ER1901-99
Rabbit Polyclonal to P2X7
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (10 µg/Lane)
Lane 3: Mouse cerebellum tissue lysate (10 µg/Lane)
Lane 4: Rat brain tissue lysate (10 µg/Lane)
Predicted band size: 69 kDa
Observed band size: 75 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-99) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
P2X7 Rabbit Polyclonal Antibody (ER1901-99)
Immunogen
Synthetic peptide corresponding to c terminal rat p2x7.
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, rat brain tissue, human kidney tissue, mouse brain tissue, THP-1.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:2000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 69 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
P2X7
SYNONYMS
ATP receptor antibody;P2rx7 antibody;P2RX7_HUMAN antibody;P2X purinoceptor 7 antibody;P2X7 antibody;P2Z receptor antibody;Purinergic receptor antibody;purinergic receptor P2X, ligand gated ion channel, 7 antibody
SEQUENCE SIMILARITIES
Belongs to the P2X receptor family.
TISSUE SPECIFICITY
Widely expressed with highest levels in brain and immune tissues.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation results in its inactivation.; ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.; Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
P2X purinoceptor 7 is a protein that in humans is encoded by the P2RX7 gene.The product of this gene belongs to the family of purinoceptors for ATP. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay criteria.The receptor is found in the central and peripheral nervous systems, in microglia, in macrophages, in uterine endometrium, and in the retina. The P2X7 receptor also serves as a pattern recognition receptor for extracellular ATP-mediated apoptotic cell death,regulation of receptor trafficking, mast cell degranulation, and inflammation. Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells
CITATIONS
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Wang, T., Li, J., Li, H., Zhong, X., Wang, L., Zhao, S., Liu, X., Huang, Z., & Wang, Y.
Aerobic Exercise Inhibited P2X7 Purinergic Receptors to Improve Cardiac Remodeling in Mice With Type 2 Diabetes
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IHC
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Mouse
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Citation
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