PRODUCT CODE: ER1901-99

P2X7 Rabbit Polyclonal Antibody (ER1901-99)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of P2X7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SHSY5Y cell lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: Rat brain tissue lysate<br />
Lane 4: Mouse brain tonsil tissue lysate
  • Western blot analysis of P2X7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SHSY5Y cell lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: Rat brain tissue lysate<br />
Lane 4: Mouse brain tonsil tissue lysate
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2X7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-99, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-P2X7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-99, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P2X7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-99, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of P2X7 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-99, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blcak).
Western blot analysis of P2X7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SHSY5Y cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Rat brain tissue lysate
Lane 4: Mouse brain tonsil tissue lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

P2X7 Rabbit Polyclonal Antibody (ER1901-99)

Immunogen

Synthetic peptide corresponding to c terminal rat p2x7.

Host

Rabbit

Positive Control

SHSY5Y cell lysate, Jurkat cell lysate, rat brain tissue lysate, mouse brain tonsil tissue lysate, rat brain tissue, human kidney tissue, mouse brain tissue, THP-1.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size 68/58/49/36 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

P2X7

SYNONYMS

ATP receptor antibody; P2rx7 antibody; P2RX7_HUMAN antibody; P2X purinoceptor 7 antibody; P2X7 antibody; P2Z receptor antibody; Purinergic receptor antibody; purinergic receptor P2X, ligand gated ion channel, 7 antibody

SEQUENCE SIMILARITIES

Belongs to the P2X receptor family.

TISSUE SPECIFICITY

Widely expressed with highest levels in brain and immune tissues.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation results in its inactivation.; ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.; Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

P2X purinoceptor 7 is a protein that in humans is encoded by the P2RX7 gene.The product of this gene belongs to the family of purinoceptors for ATP. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay criteria.The receptor is found in the central and peripheral nervous systems, in microglia, in macrophages, in uterine endometrium, and in the retina. The P2X7 receptor also serves as a pattern recognition receptor for extracellular ATP-mediated apoptotic cell death,regulation of receptor trafficking, mast cell degranulation, and inflammation. Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells