P2X7 Recombinant Rabbit Monoclonal Antibody [PSH22-44] (HA724296)
Catalog# HA724296
Rabbit Monoclonal to P2X7
Tested for
-
WB
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of P2X7 on different lysates with Rabbit anti-P2X7 antibody (HA724296) at 1/25,000 dilution.
Lane 1: Mouse brain lysate (no heat)
Lane 2: Mouse skeletal muscle lysate (no heat) (negative)
Lane 3: Mouse lung tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724296) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Low expression or negative control : Mouse skeletal muscle (PMID: 15254086, PMID: 22415881)
Western blot analysis of P2X7 on different lysates with Rabbit anti-P2X7 antibody (HA724296) at 1/25,000 dilution.
Lane 1: Mouse brain lysate (no heat)
Lane 2: Mouse skeletal muscle lysate (no heat) (negative)
Lane 3: Mouse lung tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724296) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Low expression or negative control : Mouse skeletal muscle (PMID: 15254086, PMID: 22415881)