Positive control:
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
P2X6 Rabbit Polyclonal Antibody (ER1901-49)
Immunogen
Synthetic peptide within rat p2rx6 aa 340-389 / 389.
Host
Rabbit
Positive Control
Jurkat cell lysate, K562 cell lysate, rat skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, mouse heart tissue, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
43 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB:1:500-1:2,000
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
P2X6
SYNONYMS
ATP receptor antibody; P2RX6 antibody; P2RX6_HUMAN antibody; P2RXL1 antibody; P2X purinoceptor 6 antibody; P2X6 antibody; P2XM antibody; Purinergic receptor antibody; Purinergic receptor P2X ligand gated ion channel 6 antibody; Purinergic receptor P2X like 1 antibody; Purinergic receptor P2X-like 1 antibody; Purinoceptor P2X6 antibody; Purinoreceptor P2X6 antibody; Skeletal muscle expressed P2X antibody
SEQUENCE SIMILARITIES
Belongs to the P2X receptor family.
TISSUE SPECIFICITY
Expressed predominantly in skeletal muscle.
POST-TRANSLATIONAL MODIFICATION
N-glycosylated.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
P2X purinoceptor 6 is a protein that in humans is encoded by the P2RX6 gene. The P2X receptor family is comprised of ligand-gated ion channels that allow for the increased permeability of calcium into the cell in response to extracellular ATP. The seven P2X receptors, P2X1-P2X7, form either homomeric or heteromeric channels or both. They are characterized by intracellular amino- and carboxy-termini. P2X receptors are expressed in a wide variety of tissues, including neurons, prostate, bladder, pancreas, colon, testis and ovary. The major function of the P2X receptors is to mediate synaptic transmissions between neurons and to other tissues via the binding of extracellular ATP, which acts as a neurotransmitter. The P2X receptors may be involved in the onset of necrosis or apoptosis after prolonged exposure to high concentrations of extracellular ATP. The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells.