PRODUCT CODE: ER2001-36

P2RX5 Rabbit Polyclonal Antibody (ER2001-36)

Applications

  • WB

  • IHC

REACTIVITY

  • Rat

  • Mouse

Western blot analysis of P2RX5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-36, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat brain tissue lysate<br />
Lane 2: Rat heart tissue lysate
  • Western blot analysis of P2RX5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-36, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat brain tissue lysate<br />
Lane 2: Rat heart tissue lysate
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-36, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of P2RX5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-36, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue lysate
Lane 2: Rat heart tissue lysate

Applications

  • WB

  • IHC

REACTIVITY

  • Rat

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

P2RX5 Rabbit Polyclonal Antibody (ER2001-36)

Immunogen

Synthetic peptide within rat p2rx5 aa 400-455.

Host

Rabbit

Positive Control

Rat brain tissue lysate, rat heart tissue lysate, mouse brain tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

51 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

P2RX5

SYNONYMS

ATP receptor antibody; ATP receptor subunit antibody; Ionotropic ATP receptor P2X5 antibody; LRH 1 antibody; LRH1 antibody; Lymphoid restricted histocompatibility antigen 1 antibody; MGC47755 antibody; P2rx5 antibody; P2RX5_HUMAN antibody; P2X purinoceptor 5 antibody; P2X5 antibody; P2X5R antibody; Purinergic receptor antibody; Purinergic receptor P2X ligand gated ion channel 5 antibody; Purinergic receptor P2X5 antibody

SUBCELLULAR LOCATION

Membrane.

FUNCTION

P2X purinoceptor 5 is a protein that in humans is encoded by the P2RX5 gene. The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Four transcript variants encoding distinct isoforms have been identified for this gene.