PRODUCT CODE: ET1706-43

p23 Recombinant Rabbit Monoclonal Antibody [JU09-31] (ET1706-43)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of p23 on different lysates using anti-p23 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Mouse brain tissue<br />
   Lane 2: SK-Br-3<br />
 Lane 3: Rat lung tissue
  • Western blot analysis of p23 on different lysates using anti-p23 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Mouse brain tissue<br />
   Lane 2: SK-Br-3<br />
 Lane 3: Rat lung tissue
  • ICC staining p23 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining p23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p23 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p23 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-p23 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-p23 antibody. Counter stained with hematoxylin.
Western blot analysis of p23 on different lysates using anti-p23 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain tissue
Lane 2: SK-Br-3
Lane 3: Rat lung tissue

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

p23 Recombinant Rabbit Monoclonal Antibody [JU09-31] (ET1706-43)

Immunogen

Synthetic peptide within human p23 aa 43-92 / 160.

Host

Rabbit

Positive Control

Mouse brain tissue lysate, SK-Br-3, rat lung tissue lysate, A431, Hela, human tonsil tissue, human colon cancer tissue, mouse fallopian tube tissue, rat testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU09-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

p23

SYNONYMS

Co chaperone p23 antibody; cPGES antibody; Cytosolic prostaglandin E synthase antibody; Cytosolic prostaglandin E2 synthase antibody; Hsp90 co chaperone antibody; Hsp90 co-chaperone antibody; P23 antibody; Progesterone receptor complex antibody; Progesterone receptor complex p23 antibody; Prostaglandin E synthase 3 (cytosolic) antibody; Prostaglandin E synthase 3 antibody; PTGES 3 antibody; PTGES3 antibody; Sid 3177 antibody; TEBP antibody; TEBP_HUMAN antibody; Telomerase binding protein p23 antibody; Telomerase-binding protein p23 antibody; Unactive progesterone receptor 23 kD antibody

SEQUENCE SIMILARITIES

Belongs to the p23/wos2 family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

P23, also known as PTGES3 (prostaglandin E synthase 3) or TEBP (telomerase-binding protein p23), is a ubiquitously expressed protein that functions as a cochaperone and plays an important role in signal transduction. One of several proteins in the HSP 90-based molecular chaperone complex, P23 promotes the breakdown of transcriptional regulatory complexes by disrupting receptor-mediated transcriptional activation. P23 acts in a hormone-dependent manner to chaperone estrogen receptor alpha (ERα), a steroid complex, to its mature form and to regulate the expression of ERα-related genes. Localized to the cytoplasm, P23 interacts with the glucocorticoid receptor (GR) and, through disassembly of the GR transcription machinery, is thought to inhibit GR-dependent transcription. The involvement of P23 in various steroid receptor-mediated pathways suggests close involvement in signal transduction and regulation of cellular processes. Upregulation of P23 is implicated in the invasion and metastasis of various cancers.