p21 ARC Rabbit Polyclonal Antibody (HA500005)
Catalog# HA500005
Rabbit Polyclonal to p21 ARC
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500005) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
p21 ARC Rabbit Polyclonal Antibody (HA500005)
Immunogen
Synthetic peptide within human p21 arc aa 120-178.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, rat brain tissue, human colon tissue, HUVEC.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 21 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
p21 ARC
SYNONYMS
Actin related protein 2/3 complex subunit 3 antibody;ARC21 antibody;ARP2/3 complex 21 kDa subunit antibody;ARP2/3 protein complex subunit p21 antibody;ARPC3 antibody
SUBCELLULAR LOCATION
Cell projection, Cytoplasm, Cytoskeleton, Nucleus.
FUNCTION
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (. The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility . In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA . The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) .
CITATIONS
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Tian D, Meng J, Li L, et al
Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway
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WB
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Mouse
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Citation
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The HSP90 inhibitor KW-2478 depletes the malignancy of BCR/ABL and overcomes the imatinib-resistance caused by BCR/ABL amplification
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WB
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Human
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Citation
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Resveratrol Pretreatment Improved Heart Recovery Ability of Hyperglycemic Bone Marrow Stem Cells Transplantation in Diabetic Myocardial Infarction by Down-Regulating MicroRNA-34a
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WB
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Rat
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Citation
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TPGS-1000 exhibits potent anticancer activity for hepatocellular carcinoma in vitro and in vivo
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WB
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Human
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Citation
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