p21 Recombinant Rabbit Monoclonal Antibody [PSH22-37] (HA724295)
Catalog# HA724295
Rabbit Monoclonal to p21
Tested for
-
WB
-
IF-Cell
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IHC-P
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE),Cell treatment (CT)
Western blot analysis of p21 on different lysates with Rabbit anti-p21 antibody (HA724295) at 1/2,000 dilution.
Lane 1: HCT 116 (Human colon cancer cells)
Lane 2: NCI-H1299 (Human non-small cell Lung carcinoma cells) (Negative)
Lane 3: A549 (Human lung adenocarcinoma cells)
Lane 4: MCF7 (Human breast cancer cells)
Lane 5: MCF7 (Human breast cancer cells) treated with Nutlin 3a (10uM, 24h)
Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minutes; ECL: K1801
H1299 is a negative control (PMID: 23268708).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724295, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 21 kDa
Observed band size: 21 kDa
Western blot analysis of p21 on different lysates with Rabbit anti-p21 antibody (HA724295) at 1/2,000 dilution.
Lane 1: HCT 116 (Human colon cancer cells)
Lane 2: NCI-H1299 (Human non-small cell Lung carcinoma cells) (Negative)
Lane 3: A549 (Human lung adenocarcinoma cells)
Lane 4: MCF7 (Human breast cancer cells)
Lane 5: MCF7 (Human breast cancer cells) treated with Nutlin 3a (10uM, 24h)
Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minutes; ECL: K1801
H1299 is a negative control (PMID: 23268708).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724295, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 21 kDa
Observed band size: 21 kDa