p21 Recombinant Rabbit Monoclonal Antibody [JE00-63] (HA722685)
Catalog# HA722685
Rabbit Monoclonal to p21
Tested for
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WB
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IF-Cell
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IHC-P
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IP
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of p21 on different lysates with Rabbit anti-p21 antibody (HA722685) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HUVEC cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: A549 cell lysate
Lane 6: LNCaP cell lysate
Lane 7: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 21 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722685) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HUVEC cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: A549 cell lysate
Lane 6: LNCaP cell lysate
Lane 7: 293T cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 21 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722685) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.