PRODUCT CODE: ET1602-9

p16 ARC Recombinant Rabbit Monoclonal Antibody [SR34-02] (ET1602-9)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1,000 dilution.<br />
Positive control: <br />
  Lane 1: MCF-7 <br />
  Lane 2: SK-BR-3
  • Western blot analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1,000 dilution.<br />
Positive control: <br />
  Lane 1: MCF-7 <br />
  Lane 2: SK-BR-3
  • ICC staining p16 ARC in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with p16 ARC antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.
Western blot analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: SK-BR-3

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

p16 ARC Recombinant Rabbit Monoclonal Antibody [SR34-02] (ET1602-9)

Immunogen

Synthetic peptide within human p16 arc n terminal.

Host

Rabbit

Positive Control

N2A, MCF-7, SK-BR-3, human spleen tissue, mouse lung tissue, mouse spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR34-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

16 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50 IHC-P

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

p16 ARC

SYNONYMS

Actin related protein 2/3 complex subunit 5 (16 kD) antibody; Actin related protein 2/3 complex subunit 5 antibody; Actin related protein 2/3 complex, subunit 5 16kDa antibody; Actin-related protein 2/3 complex subunit 5 antibody; ARC16 antibody; Arp2/3 complex 16 kDa subunit antibody; Arp2/3 protein complex subunit p16 antibody; ARPC 5 antibody; Arpc5 antibody; ARPC5_HUMAN antibody; dJ127C7.3 antibody; MGC88523 antibody; p16 Arc antibody; p16-ARC antibody; RP1 127C7.3 antibody

SEQUENCE SIMILARITIES

Belongs to the ARPC5 family.

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm

FUNCTION

The Arp2/3 (Actin-related protein 2/3) complex consists of seven subunits, all of which are actin-related proteins. The complex is involved in the control of actin polymerization and in mediating the formation of branched actin networks. p16-ARC, also known as ARPC5 (Actin-related protein 2/3 complex subunit 5) or ARC16 (Arp2/3 complex 16 kDa subunit), is a 151 amino acid subunit of the Arp2/3 complex. Thought to play a role in maintaining the integrity of Arp2/3, p16-ARC is a substrate for MAPKAPK-2 which, through phosphorylation of p16-ARC, may participate in Arp2/3 regulatory functions and remodeling of the Actin cytoskeleton. Two isoforms of p16-ARC exist due to alternative splicing events.