p16INK4a Recombinant Mouse Monoclonal Antibody [PD01-16] (HA601131)
Catalog# HA601131
Mouse Monoclonal to p16
Tested for
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IHC-P
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WB
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IF-Cell
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-p16INK4a antibody (HA601131) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601131) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601131) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.