PRODUCT CODE: ER1901-75

p15 INK4b Rabbit Polyclonal Antibody (ER1901-75)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of p15 INK4b on recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-75, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of p15 INK4b on recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-75, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining p15 INK4b in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1901-75) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining p15 INK4b in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1901-75) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-p15 INK4b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-75) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-p15 INK4b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-75) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of p15 INK4b was done on Hela cells. The cells were fixed, permeabilized and stained with p15 INK4b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-Rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Western blot analysis of p15 INK4b on recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-75, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

p15 INK4b Rabbit Polyclonal Antibody (ER1901-75)

Immunogen

Recombinant protein within human p15 ink4b aa 1-138 / 138.

Host

Rabbit

Positive Control

Hela, SiHa, rat kidney tissue, human esophagus cancer tissue, human small intestine tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size 15 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500

  • ICC:1:50-1:200

  • IHC-P:1:50-1:200

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

p15 INK4b

SYNONYMS

CDK inhibitory protein antibody; CDK4B Inhibitor antibody; Cdkn2b antibody; CDN2B_HUMAN antibody; Cyclin dependent kinase 4 inhibitor B antibody; Cyclin Dependent Kinase Inhibitor 2B antibody; Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4 antibody; Cyclin dependent kinases 4 and 6 binding protein antibody; Cyclin-dependent kinase 4 inhibitor B antibody; INK4B antibody; MTS 2 antibody; MTS-2 antibody; MTS2 antibody; Multiple tumor suppressor 2 antibody; Multiple Tumor Supressor 2 antibody; OTTHUMP00000021154 antibody; OTTHUMP00000021155 antibody; p14 CDK inhibitor antibody; p14 INK4b antibody; p14-INK4b antibody; P15 antibody; p15 CDK inhibitor antibody; p15 inhibits CDK4 antibody; p15 INK4b antibody; p15-INK4b antibody; p15INK4B antibody; TP 15 antibody; TP15 antibody

SEQUENCE SIMILARITIES

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

TISSUE SPECIFICITY

Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.

SUBCELLULAR LOCATION

Cytoplasm. Also found in the nucleus.

FUNCTION

Cyclin-dependent kinase 4 inhibitor B also known as multiple tumor suppressor 2 (MTS-2) or p15INK4b is a protein that is encoded by the CDKN2B gene in humans. This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, also known as p15Ink4b protein, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases by cyclin D, thus the encoded protein functions as a cell growth regulator that inhibits cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.