P Glycoprotein Recombinant Rabbit Monoclonal Antibody [SN06-42] (ET1611-30)
Catalog# ET1611-30
Rabbit Monoclonal to P Glycoprotein
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WB
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IHC-P
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IF-Tissue
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HepG2 cell lysate (no heat) (20 µg/Lane)
Lane 2: HepG2 cell lysate
Notice: no heat means the lysate is not boiled.
Predicted band size: 141 kDa
Observed band size: 150~250 kDa
Exposure time: 2 minutes 37 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-30) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
P Glycoprotein Recombinant Rabbit Monoclonal Antibody [SN06-42] (ET1611-30)
Immunogen
Recombinant protein within human p-glycoprotein 1 aa 373-730 / 1,280.
Host
Rabbit
Positive Control
HepG2 cell lysate, human liver tissue, human kidney tissue, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN06-42
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:50-1:200
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IF-Tissue
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1:50
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IF-Cell:1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 141 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
P Glycoprotein
SYNONYMS
ABC20 antibody;ABCB1 antibody;ATP binding cassette, sub family B (MDR/TAP), member 1 antibody;ATP-binding cassette sub-family B member 1 antibody;CD243 antibody;CLCS antibody;Colchicin sensitivity antibody;Doxorubicin resistance antibody;GP170 antibody;MDR1 antibody;MDR1_HUMAN antibody;Multidrug resistance 1 antibody;Multidrug resistance protein 1 antibody;P glycoprotein 1 antibody;P gp antibody;P-glycoprotein 1 antibody;PGY1 antibody
SEQUENCE SIMILARITIES
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
TISSUE SPECIFICITY
Expressed in liver, kidney, small intestine and brain.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation 243 (CD243) is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent efflux pump with broad substrate specificity. It exists in animals, fungi, and bacteria, and it likely evolved as a defense mechanism against harmful substances. P-gp is extensively distributed and expressed in the intestinal epithelium where it pumps xenobiotics (such as toxins or drugs) back into the intestinal lumen, in liver cells where it pumps them into bile ducts, in the cells of the proximal tubule of the kidney where it pumps them into urinary filtrate (in the proximal tubule), and in the capillary endothelial cells composing the blood–brain barrier and blood-testis barrier, where it pumps them back into the capillaries.
CITATIONS
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Jun Wang , Wenjia Liang , Xiangwen Wang , Zhao Chen , Lei Jiang
LTBP2 regulates cisplatin resistance in GC cells via activation of the NF-κB2/BCL3 pathway
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WB
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Human
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Citation
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Mengyuan Li , Shuwen Zheng , Qinyuan Gong , Haifeng Zhuang , Zhaoxing Wu , Ping Wang , Xuzhao Zhang , Rongzhen Xu
An oral triple pill-based cocktail effectively controls acute myeloid leukemia with high translation
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WB
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Human
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Citation
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