Osteopontin Rabbit Polyclonal Antibody (ER1802-16)
Catalog# ER1802-16
Rabbit Polyclonal to Osteopontin
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IF-Cell
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IHC-P
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ELISA
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Human
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Mouse
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Osteopontin Rabbit Polyclonal Antibody (ER1802-16)
Immunogen
Synthetic peptide within mouse osteopontin 17-100.
Host
Rabbit
Positive Control
HepG2, MCF-7, human liver cancer tissue, human kidney tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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ELISA
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1:5,000-1:10,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Osteopontin
SYNONYMS
BNSP antibody;Bone sialoprotein 1 antibody;BSP I antibody;BSPI antibody;Early T lymphocyte activation 1 antibody;ETA 1 antibody;ETA1 antibody;MGC110940 antibody;Nephropontin antibody;OPN antibody;Osteopontin antibody;osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody;OSTP_HUMAN antibody;PSEC0156 antibody;secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody;Secreted phosphoprotein 1 antibody;SPP 1 antibody;SPP-1 antibody;SPP1 antibody;SPP1/CALPHA1 fusion antibody;Urinary stone protein antibody;Uropontin antibody
SEQUENCE SIMILARITIES
Belongs to the osteopontin family.
TISSUE SPECIFICITY
Bone. Found in plasma.
POST-TRANSLATIONAL MODIFICATION
Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.
CITATIONS
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Qing Wang,et al
S100A9 promotes renal calcium oxalate stone formation via activating the TLR4-p38/MAPK-LCN2 signaling pathway
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IHC
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Mouse
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Citation
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Single-Cell RNA Sequencing Unravels Distinct Tumor Microenvironment of Different Components of Lung Adenocarcinoma Featured as Mixed Ground-Glass Opacity
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ICC
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Human
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Citation
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Peng Zhiwei,et al
Crosstalk between cancer-associated fibroblasts and myeloid cells shape the heterogeneous microenvironment of gastric cancer
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IF
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Human
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Citation
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Xuewu You ,et al
Single-cell characterization of intra-tumoral heterogeneity and the immunosuppressive microenvironment in uterine serous carcinoma
