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PRODUCT CODE: 0806-6

Osteopontin Rabbit Polyclonal Antibody (0806-6)

Applications

  • WB

REACTIVITY

  • Mouse

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Western blot analysis of Osteopontin on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0806-6, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Osteopontin on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0806-6, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Osteopontin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0806-6, 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Rat kidney tissue lysate<br /> Lane 2: Mouse kidney tissue lysate<br /> Lane 3: HepG2 cell lysate
Western blot analysis of Osteopontin on mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0806-6, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Osteopontin Rabbit Polyclonal Antibody (0806-6)

Immunogen

Synthetic peptide within mouse osteopontin aa 245-294 / 294.

Host

Rabbit

Positive Control

Mouse liver tissue lysates, rat kidney tissue lysate, mouse kidney tissue lysate, HepG2 cell lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

TARGET

UNIPROT #

P10451

PROTEIN NAME

Osteopontin

SYNONYMS

BNSP antibody; Bone sialoprotein 1 antibody; BSP I antibody; BSPI antibody; Early T lymphocyte activation 1 antibody; ETA 1 antibody; ETA1 antibody; MGC110940 antibody; Nephropontin antibody; OPN antibody; Osteopontin antibody; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody; OSTP_HUMAN antibody; PSEC0156 antibody; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody; Secreted phosphoprotein 1 antibody; SPP 1 antibody; SPP-1 antibody; SPP1 antibody; SPP1/CALPHA1 fusion antibody; Urinary stone protein antibody; Uropontin antibody

SEQUENCE SIMILARITIES

Belongs to the osteopontin family.

POST-TRANSLATIONAL MODIFICATION

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated.

SUBCELLULAR LOCATION

Cytosol.

FUNCTION

Osteopontin is an extracellular matrix cell adhesion protein which is abundant in bone and synthesized by preosteoblasts, osteoblasts and osteoclastic cells that are localized in the mineralized phase of bone matrix. Osteopontin probably exists in tissue-specific isoforms that may correspond to particular cellular functions. Acts as a cytokine it involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

CITATIONS

  • Lin, Chujiao et al.

    Circulating miR-338 Cluster activities on osteoblast differentiation: Potential Diagnostic and Therapeutic Targets for Postmenopausal Osteoporosis. | Theranostics [2019]

  • Li, T., Wang, H., Liu, R......

    Li, T., Wang, H., Liu, R., Wang, X., Huang, L., Wu, Z., Yin, X., Zou, S., & Duan, P. (2021). The role of EphB4/ephrinB2 signaling in root repair after orthodontically-induced root resorption. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics, 159(3), e217–e232.

  • Yu, S., Guo, J., Sun, Z.......

    Yu, S., Guo, J., Sun, Z., Lin, C., Tao, H., Zhang, Q., Cui, Y., Zuo, H., Lin, Y., Chen, S., Liu, H., & Chen, Z. (2021). BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation. Cell death & disease, 12(2), 197.

  • Shen, Jian et al.

    Regulation of Vascular Calcification by Growth Hormone-Releasing Hormone and Its Agonists. | Circulation Research [2018]

  • Liu, Q., Xiang, P., Chen......

    Liu, Q., Xiang, P., Chen, M., Luo, Y., Zhao, Y., Zhu, J., Jing, W., & Yu, H. (2021). Nano-Sized Hydroxyapatite Induces Apoptosis and Osteogenic Differentiation of Vascular Smooth Muscle Cells via JNK/c-JUN Pathway. International journal of nanomedicine, 16, 3633–3648.