Applications
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WB
REACTIVITY
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Human
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Rat
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Mouse
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Osteopontin Rabbit Polyclonal Antibody (0806-6)
Immunogen
Synthetic peptide within mouse osteopontin aa 245-294 / 294.
Host
Rabbit
Positive Control
Mouse liver tissue lysates, rat kidney tissue lysate, mouse kidney tissue lysate, HepG2 cell lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
32 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:500-1:2000
TARGET
UNIPROT #
PROTEIN NAME
Osteopontin
SYNONYMS
BNSP antibody; Bone sialoprotein 1 antibody; BSP I antibody; BSPI antibody; Early T lymphocyte activation 1 antibody; ETA 1 antibody; ETA1 antibody; MGC110940 antibody; Nephropontin antibody; OPN antibody; Osteopontin antibody; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody; OSTP_HUMAN antibody; PSEC0156 antibody; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody; Secreted phosphoprotein 1 antibody; SPP 1 antibody; SPP-1 antibody; SPP1 antibody; SPP1/CALPHA1 fusion antibody; Urinary stone protein antibody; Uropontin antibody
SEQUENCE SIMILARITIES
Belongs to the osteopontin family.
POST-TRANSLATIONAL MODIFICATION
Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated.
SUBCELLULAR LOCATION
Cytosol.
FUNCTION
Osteopontin is an extracellular matrix cell adhesion protein which is abundant in bone and synthesized by preosteoblasts, osteoblasts and osteoclastic cells that are localized in the mineralized phase of bone matrix. Osteopontin probably exists in tissue-specific isoforms that may correspond to particular cellular functions. Acts as a cytokine it involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
CITATIONS
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Yu, S., Guo, J., Sun, Z., L...
BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation. Cell death & disease, 12(2), 197.
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IHC
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Mouse
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Citation
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Shen, Jian et al.
Regulation of Vascular Calcification by Growth Hormone-Releasing Hormone and Its Agonists. | Circulation Research [2018]
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WB
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Vascular SMCs
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Citation
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Liu, Q., Xiang, P., Chen, M...
Nano-Sized Hydroxyapatite Induces Apoptosis and Osteogenic Differentiation of Vascular Smooth Muscle Cells via JNK/c-JUN Pathway. International journal of nanomedicine, 16, 3633–3648.
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WB
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Mouse
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Citation
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Lin, Chujiao et al.
Circulating miR-338 Cluster activities on osteoblast differentiation: Potential Diagnostic and Therapeutic Targets for Postmenopausal Osteoporosis. | Theranostics [2019]
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Li, T., Wang, H., Liu, R., ...
The role of EphB4/ephrinB2 signaling in root repair after orthodontically-induced root resorption. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics, 159(3), e217–e232.
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Rat
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Citation
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