PRODUCT CODE: ER1803-11

Orai1 Rabbit Polyclonal Antibody (ER1803-11)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution.
  • Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution.
  • ICC staining Orai1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Orai1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- Orai1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Orai1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with Orai1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Orai1 Rabbit Polyclonal Antibody (ER1803-11)

Immunogen

Synthetic peptide within mouse orai1 aa 181-230 / 304.

Host

Rabbit

Positive Control

PC-3M, LOVO, MCF-7, human skin tissue, human esophagus tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Orai1

SYNONYMS

Calcium release activated calcium channel protein 1 antibody; Calcium release activated calcium modulator 1 antibody; Calcium release-activated calcium channel protein 1 antibody; CRACM 1 antibody; CRACM1 antibody; CRCM1 antibody; CRCM1_HUMAN antibody; FLJ14466 antibody; Orai 1 antibody; ORAI calcium release activated calcium modulator 1 antibody; orai1 antibody; ORAT1 antibody; Protein orai 1 antibody; Protein orai-1 antibody; TMEM 142A antibody; TMEM142A antibody; Transmembrane protein 142A antibody

SEQUENCE SIMILARITIES

Belongs to the Orai family.

TISSUE SPECIFICITY

Expressed in naive CD4 and CD8 T cells (at protein level). Expressed at similar levels in naive and effector T helper cells.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. N-glycosylation inhibits channel activity in T cells.; Ubiquitinated.; Cys-195 is oxidated, leading to inactivation of channel activity.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Orai1 (ORAI calcium release-activated calcium modulator 1), also known as ORAT1, CRACM1 (calcium release-activated calcium modulator 1) or TMEM142A (transmembrane protein 142A), is a 301 amino acid multi-pass membrane protein that belongs to the Orai family of proteins. Localizing to the plasma membrane, Orai1 plays an important role in store-operated calcium (SOC) entry, a process involving Ca2+ influx and replenishment of Ca2+ stores formerly emptied through the action of inositol 1,4,5-trisphosphate production and other Ca2+ mobilizing agents. Specifically, Orai1 functions as a pore subunit of the store-operated calcium release-activated calcium channel (CRAC) and is essential for proper function of the CRAC channel. CRAC channels are responsible for mediating calcium influx in T-cells and play an important role in the immune response. Mutations in the gene encoding Orai1 can result in severe combined immunodeficiency (SCID).